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KMID : 0880220060440060689
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Journal of Microbiology
2006 Volume.44 No. 6 p.689 ~ p.693
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Carbon Source-Dependent Regulation of the Schizosaccharomyces pombe pbh1 Gene
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Kim Su-Jung
Cho Nam-Chul
Ryu In-Wang
Kim Kyung-Hoon
Park Eun-Hee
Lim Chang-Jin
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Abstract
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Pbh1, from the fission yeast Schizosaccharomyces pombe, is a baculoviral inhibitor of apoptosis (IAP) repeat (BIR) domain-containing protein. Its unique encoding gene was previously found to be regulated by nitric oxide and nitrogen starvation. In the current work, the Pbh1-lacZ fusion gene was used to elucidate the transcriptional regulation of the pbh1 gene under various carbon sources. When fermentable carbon sources, such as glucose (at a low concentration of 0.2%), sucrose (2.0%) and lactose (2.0%), were the sole carbon source, the synthesis of β-galactosidase from the Pbh1-lacZ fusion gene was reasonably enhanced. However, the induction by these fermentable carbon sources was abolished in the Pap1-negative S. pombe cells, implying that this type of induction of the pbh1 gene is mediated by Pap1. Ethanol (2.0%), a nonfermentable carbon source, was also able to enhance the synthesis of β-galactosidase from the fusion gene in wild-type cells but not in Pap1-negative cells. The results indicate that the S. pombe pbh1 gene is up-regulated under metabolic oxidative stress in a Pap1-dependent manner.
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KEYWORD
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carbon source, fission yeast, Pap1, Pbh1, Schizosaccharomyces pombe, transcriptional regulation
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